| 货号 | 6344S |
| 描述 | SignalSilence® CREB siRNA II (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit CREB expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Mouse |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9). |
| 存放说明 | -20C |
| 参考文献 | Lonze, B.E. et al. (2002) Neuron 34, 371-85. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12. Redmond, L. et al. (2002) Neuron 34, 999-1010. Dash, P.K. et al. (1990) Nature 345, 718-21. Yin, J.C. et al. (1994) Cell 79, 49-58. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55. Ribar, T.J. et al. (2000) J Neurosci 20, RC107. Tan, Y. et al. (1996) EMBO J 15, 4629-42. |
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® CREB siRNA I (Mouse Specific) #6343 (+) or SignalSilence® CREB siRNA II (Mouse Specific) (+), using CREB (48H2) Rabbit mAb #9197 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The CREB (48H2) Rabbit mAb confirms silencing of CREB expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot分析转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)、SignalSilence® CREB siRNA I (Mouse Specific) #6343 (+) 或 SignalSilence® CREB siRNA II (Mouse Specific) (+)的NIH/3T3细胞提取物。CREB (48H2) Rabbit mAb 兔单抗证实了CREB的表达被沉默，α-Tubulin (11H10)Rabbit mAb 兔单抗用作上样对照。 |
