| 货号 | 6302S |
| 描述 | SignalSilence® FoxO3a siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit FoxO3a expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). |
| 存放说明 | -20C |
| 参考文献 | Anderson, M.J. et al. (1998) Genomics 47, 187-99. Galili, N. et al. (1993) Nat Genet 5, 230-5. Borkhardt, A. et al. (1997) Oncogene 14, 195-202. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83. Guo, S. et al. (1999) J Biol Chem 274, 17184-92. Seoane, J. et al. (2004) Cell 117, 211-23. Arden, K.C. (2004) Mol Cell 14, 416-8. Yang, Y. et al. (2005) EMBO J 24, 1021-32. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® FoxO3a siRNA I (+) or SignalSilence® FoxO3a siRNA II #6303 (+), using FoxO3a (75D8) Rabbit mAb #2497 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The FoxO3a (75D8) Rabbit mAb confirms silencing of FoxO3a expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot 分析采用100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® FoxO3a siRNA I (+)或SignalSilence ® FoxO3a siRNA II #6303 (+)转染后的Hela细胞提取物，所用抗体为FoxO3a (75D8) Rabbit mAb 兔单抗#2497 (上) 或 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125 (下) 。 FoxO3a (75D8) Rabbit mAb兔单抗抗体确定了FoxO3a表达的沉默， α-Tubulin (11H10) Rabbit mAb兔单抗作为对照。 |
