| 货号 | 12473S |
| 描述 | SignalSilence® Fyn siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Fyn expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® Fyn siRNA I inhibits human and monkey Fyn expression. |
| 供应商 | CST |
| 背景 | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| 存放说明 | -20C |
| 参考文献 | Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609. Hunter, T. (1987) Cell 49, 1-4. Resh, M.D. (1998) Int J Biochem Cell Biol 30, 1159-62. Nel, A.E. (2002) J Allergy Clin Immunol 109, 758-70. Fukai, I. et al. (2000) Eur J Immunol 30, 3507-15. |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Fyn siRNA I (+), using Fyn Antibody #4023 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Fyn Antibody confirms silencing of Fyn expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.Western blot分析293T细胞的细胞提取物，转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或SignalSilence® Fyn siRNA I (+),使用抗体是Fyn Antibody #4023 (上图)或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。Fyn Antibody确认Fyn的表达沉默，β-Actin (D6A8) Rabbit mAb用来检测内参。 |
