| 货号 | 6312S |
| 描述 | SignalSilence® GSK-3α siRNA I from Cell Signaling Technology allows the researcher to specifically inhibit GSK-3α expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines. |
| 反应种属 | Human/Monkey |
| 应用 | TFN |
| 目标/特异性 | GSK-3α siRNA I will inhibit human, mouse, rat and monkey GSK-3α expression. |
| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).Small Interfering RNA (siRNA) has been used to specifically silence GSK-3α expression, causing an upregulation of β-catenin protein levels (6). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
| 参考文献 | Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41. Cross, D.A. et al. (1995) Nature 378, 785-9. Nusse, R. (1997) Cell 89, 321-3. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. Yu, J. Y. et al. (2003) Mol. Ther. 7, 228-236. |
Western blot analysis of extracts from HeLa cells, untransfected or transfected with either GSK-3alpha/beta siRNA #6301 or GSK-3alpha siRNA. GSK-3alpha and GSK-3beta were detected using a GSK-3alpha/beta antibody, and eIF4B was detected using eIF4B Antibody #3592. The GSK-3alpha/beta antibody confirms silencing of GSK-3alpha and beta expression, and the eIF4B Antibody is used to control for loading and siRNA specificity. |
