| 货号 | 6301S |
| 描述 | SignalSilence® GSK-3α/β siRNA from Cell Signaling Technology allows the researcher to specifically inhibit GSK-3aα and GSK-3bβ expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | GSK-3α/β siRNA will inhibit human, mouse and rat GSK-3α/β expression. |
| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Small Interfering RNA (siRNA) has been used to specifically silence GSK-3alpha and beta expression, causing an upregulation of beta-catenin protein levels (6). |
| 存放说明 | -20C |
| 计算分子量 | 46 |
| 参考文献 | Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41. Cross, D.A. et al. (1995) Nature 378, 785-9. Nusse, R. (1997) Cell 89, 321-3. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. Yu, J. Y. et al. (2003) Mol. Ther. 7, 228-236. |
Western blot analysis of extracts from HeLa cells, untransfected or transfected with GSK-3a/β siRNA. GSK-3β was detected using GSK-3β Antibody #9332, and Bcl-xL was detected using Bcl-xL Antibody #2762. The GSK-3β Antibody confirms silencing of GSK-3β expression, and the Bcl-xL Antibody is used to control for loading and siRNA specificity. | |
Western blot 分析HeLa细胞提取物，分为未转染组和GSK-3α/β siRNA #6301 转染组， GSK-3β检测所用抗体为GSK-3β Antibody #9332， Bcl-xL检测采用Bcl-xL Antibody #2762 。 GSK-3β Antibody验证了 GSK-3β表达的沉默， Bcl-xL Antibody作为上样和siRNA的特异性的对照。 |
