| 货号 | 7595S |
| 描述 | SignalSilence® HDAC4 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit HDAC4 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® HDAC4 siRNA I will inhibit human, mouse and rat HDAC4 exoression. |
| 供应商 | CST |
| 背景 | Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). |
| 存放说明 | -20C |
| 参考文献 | Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53. Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16. Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83. Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® HDAC4 siRNA I (+) or SignalSilence® HDAC4 siRNA II #7609 (+), using HDAC4 (D15C3) Rabbit mAb #7628 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The HDAC4 (D15C3) Rabbit mAb confirms silencing of HDAC4 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. 使用HDAC4 (D15C3) Rabbit mAb #7628兔单抗 (上图)或α-Tubulin (11H10) Rabbit mAb #2125 兔单抗(下图)，免疫印迹（Western blot）分析HeLa细胞中HDAC4蛋白水平，细胞分别转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)、SignalSilence® HDAC4 siRNA I (+)或SignalSilence® HDAC4 siRNA II #7609 (+)。HDAC4 (D15C3) Rabbit mAb确定HDAC4基因沉默，然而α-Tubulin (11H10) Rabbit mAb通常用做内参。 |
