| 货号 | 6282S |
| 描述 | SignalSilence® HER2/ErbB2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit HER2/ErbB2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
| 存放说明 | -20C |
| 参考文献 | Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57. Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4. Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4. Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8. Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® HER2/ErbB2 siRNA I (+) or SignalSilence® HER2/ErbB2 siRNA II #6283 (+), using HER2/ErbB2 (29D8) Rabbit mAb #2165 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The HER2/ErbB2 (29D8) Rabbit mAb confirms silencing of HER2/ErbB2 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. 用抗体HER2/ErbB2 (29D8) Rabbit mAb #2165（上）和α-Tubulin (11H10) Rabbit mAb #2125（下）对经过以下处理的Hela细胞进行免疫印迹检测：100 nM SignalSilence® Control siRNA(未结合的) #6568 (-)，SignalSilence® HER2/ErbB2 siRNA I (+)或者SignalSilence® HER2/ErbB2 siRNA II #6283 (+)转染的 HeLa细胞。抗HER2/ErbB2 (29D8) Rabbit mAb确定了对HER2/ErbB2表达的沉默作用，而α-Tubulin (11H10) Rabbit mAb作为对照确定上样量。 |
