| 货号 | 14178S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM SignalSilence® Hexokinase II siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). |
| 存放说明 | -20C |
| 参考文献 | WARBURG, O. (1956) Science 123, 309-14. Semenza, G.L. (2000) Crit Rev Biochem Mol Biol 35, 71-103. Smith, T.A. (2000) Br J Biomed Sci 57, 170-8. |
Western blot analysis of extracts from MCF7 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Hexokinase II siRNA I (+), using Hexokinase II (C64G5) Rabbit mAb #2867 (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The Hexokinase II (C64G5) Rabbit mAb confirms silencing of hexokinase II expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control. |
