| 货号 | 7668S |
| 描述 | SignalSilence® hnRNP A1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit hnRNP A1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). hnRNP A1 regulates the alternative splicing of c-Src and c-H-Ras (2,3) and modifies initiation of translation of the fibroblast growth factor 2 mRNA (4). hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells (5). Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus (6,7). hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production (8,9). HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm (10). |
| 存放说明 | -20C |
| 参考文献 | Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82. Rooke, N. et al. (2003) Mol Cell Biol 23, 1874-84. Guil, S. et al. (2003) Mol Cell Biol 23, 2927-41. Bonnal, S. et al. (2005) J Biol Chem 280, 4144-53. Patry, C. et al. (2003) Cancer Res 63, 7679-88. Piñol-Roma, S. and Dreyfuss, G. (1992) Nature 355, 730-2. Siomi, M.C. et al. (1997) J Cell Biol 138, 1181-92. Black, A.C. et al. (1996) Virus Genes 12, 275-85. Hadian, K. et al. (2009) J Biol Chem 284, 33384-91. Monette, A. et al. (2009) J Biol Chem 284, 31350-62. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® hnRNP A1 siRNA I (+), using hnRNP A1 (D21H11) Rabbit mAb #8443 and α-Tubulin (11H10) Rabbit mAb #2125. The hnRNP A1 (D21H11) Rabbit mAb confirms silencing of hnRNP A1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot 方法检测HeLa细胞提取物。用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® hnRNP A1 siRNA I (+)转染细胞。所用抗体为hnRNP A1 (D21H11) Rabbit mAb兔单抗 #8443 或 α-Tubulin (11H10) Rabbit mAb兔单抗 #2125。hnRNP A1 (D21H11) Rabbit mAb兔单抗确定hnRNP A1表达的沉默效率，α-Tubulin (11H10) Rabbit mAb兔单抗作为上样量对照。 |
