| 货号 | 6356S |
| 描述 | SignalSilence® HSP27 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit HSP27 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
| 参考文献 | Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803. Rouse, J. et al. (1994) Cell 78, 1027-1037. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177. Park, K. et al. (2003) J. Biol. Chem. 278, 35272-35278. |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. 活Lela细胞转染SignalSilence®Control siRNA (Fluorescein Conjugate) #620124小时后，荧光检测显示约100%的转染效率。 | |
Western blot analysis of extracts from HeLa cells transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or HSP27 siRNA I (+), using HSP27 (G31) Mouse mAb #2402 and p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107. The HSP27 (G31) mAb confirms silencing of HSP27 expression and p44/42 MAPK mAb is used to control for loading and specificity of HSP27 siRNA. 对转染100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)或HSP27 siRNA I (+)的HeLa细胞使用HSP27 (G31) Mouse mAb #2402和p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107进行Western blot分析。HSP27 (G31) mAb证实HSP27的表达沉默，p44/42 MAPK mAb用作显示HSP27 siRNA上样和特异性的对照。 |
