| 货号 | 6526S |
| 描述 | SignalSilence® HSP27 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit HSP27 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). |
| 存放说明 | -20C |
| 参考文献 | Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803. Rouse, J. et al. (1994) Cell 78, 1027-1037. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® HSP27 siRNA II #6526 (+), using HSP27 (G31) Mouse mAb #2402 and α-Tubulin (11H10) Rabbit mAb #2125. HSP27 (G31) Mouse mAb confirms silencing of HSP27 expression, while α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of HSP27 siRNA. 对转染100 nM SignalSilence® Control siRNA (荧光素接合) #6201 (-)、 SignalSilence® HSP27 siRNA II #6526 (+)的HeLa细胞使用HSP27 (G31) Mouse mAb #2402和α-Tubulin (11H10)兔单克隆抗体#2125进行Western blot分析。HSP27 (G31) Mouse mAb证实HSP27的表达沉默，α-Tubulin (11H10)兔单抗用作显示HSP27 siRNA上样和特异性的对照。 |
