| 货号 | 12482S |
| 描述 | SignalSilence® IGF-I Receptor β siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit IGF-I Receptor β expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Mouse |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | -20C |
| 参考文献 | Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93. Baserga, R. (2000) Oncogene 19, 5574-81. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60. Baserga, R. (1999) Exp Cell Res 253, 1-6. White, M.F. et al. (1985) J Biol Chem 260, 9470-8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80. |
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® IGF-I Receptor β siRNA I (Mouse Specific) (+), or SignalSilence® IGF-I Receptor β siRNA II (Mouse Specific) #12595 (+), using IGF-I Receptor β (D23H3) XP® Rabbit mAb #9750 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The IGF-I Receptor β (D23H3) XP® Rabbit mAb confirms silencing of IGF-I receptor β expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. Western blot方法检测NIH/3T3细胞提取物，用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，IGF-I Receptor β siRNA I (Mouse Specific) (+)或SignalSilence® IGF-I Receptor β siRNA II (Mouse Specific) #12595 (+)转染，使用的抗体为IGF-I Receptor β (D23H3) XP® Rabbit mAb兔单抗 #9750 (上图) 或β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下图). 当β-Actin (D6A8) Rabbit mAb 兔单抗作为内参使用时，IGF-I Receptor β (D23H3) XP® Rabbit mAb兔单抗确定了IGF-IRβ表达的沉默。 |
