| 货号 | 6372S |
| 描述 | SignalSilence® IKKα siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit IKKα expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | IKKα siRNA I will inhibit human, mouse, rat and monkey IKKα expression. |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13). |
| 存放说明 | -20C |
| 参考文献 | Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. Brown, K. et al. (1995) Science 267, 1485-8. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. Chen, Z.J. et al. (1996) Cell 84, 853-62. Zandi, E. et al. (1997) Cell 91, 243-52. Karin, M. (1999) Oncogene 18, 6867-74. DiDonato, J.A. et al. (1997) Nature 388, 548-54. Mercurio, F. et al. (1997) Science 278, 860-6. Johnson, L.N. et al. (1996) Cell 85, 149-58. Delhase, M. et al. (1999) Science 284, 309-13. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® IKKα siRNA I (+) or SignalSilence® IKKα siRNA II #6373 (+), using IKKα Antibody #2682 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The IKKα Antibody confirms silencing of IKKα expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. |
