| 货号 | 6202S |
| 描述 | SignalSilence® ILK1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ILK1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | ILK1 siRNA I will inhibit human, mouse and rat ILK1 expression. |
| 供应商 | CST |
| 背景 | Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility (1). ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin (1). ILK phosphorylates Akt at Ser473, GSK-3 on Ser9, myosin light chain 2 (MLC2) on Ser18/Thr19, as well as affixin (2-5). These phosphorylation events are key regulatory steps in modulating the activities of the targets. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN and a PP2C protein phosphatase, ILKAP (1,3,6). It has been suggested that the conserved Ser343 residue in the activation loop plays a key role in the activation of ILK1 (2). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
| 参考文献 | Wu, C. and Dedhar, S. (2000) J. Biol. Chem. 155, 505-510. Persad, S. et al. (2001) J. Biol. Chem. 276, 27462-27469. Persad, S. et al. (2000) J. Cell Biol. 153, 1161-1173. Deng, J.T. et al. (2001) J. Biol. Chem. 276, 16365-16373. Yamaji, S. et al. (2001) J. Cell Biol. 153, 1251-1264. Morimoto, A.M. et al. (2000) Oncogene 19, 200-209. Troussard, A. A. et al. (2003) J. Biol. Chem. 278(25) , 22374-22378. |
Western blot analysis of extracts from HeLa cells, transfected with 50 nM non-targeted siRNA (-) or 100 nM SignalSilence® ILK1 siRNA I (+), using ILK1 Antibody #3862 and Cofilin Antibody #3312. The ILK1 antibody confirms silencing of ILK1 expression and the cofilin antibody controls for protein loading and specificity of ILK1 siRNA.转染了50 nM非定向siRNA（-）或转染了100 nM SignalSilence® ILK1 siRNA I (+)HeLa细胞提取物进行westernblot分析，使用ILK1 抗体 #3862 和Cofilin抗体 #3312. ILK1 抗体用以确认ILK1的沉默，cofilin抗体用以作为上样量和ILK1 siRNA特异性的对照。 | |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.使用SignalSilence® 对照siRNA（荧光标记）#6201在有生命的HeLa细胞中转染24小时候，进行荧光检测，证明转染效率近100%。 |
