SignalSilence® IRF-3 siRNA I
货号：
6274S 基本售价：
3318.0 元 规格：
300ul
产品信息
概述| 货号 | 6274S |
| 描述 | SignalSilence® IRF-3 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit IRF-3 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
性能| 供应商 | CST |
| 背景 | Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2). |
| 存放说明 | -20C |
| 参考文献 | Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
|
参考图片Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® IRF-3 siRNA I (+), using IRF-3 (D83B9) Rabbit mAb #4302 and α-Tubulin (11H10) Rabbit mAb #2125. The IRF-3 (D83B9) Rabbit mAb confirms silencing of IRF-3 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western免疫印迹。用IRF-3 (D83B9) Rabbit mAb #4302 或α-Tubulin (11H10) Rabbit mAb #2125 研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)或SignalSilence® IRF-3 siRNA I (+)的K-562 细胞的细胞提取液。IRF-3 (D83B9) Rabbit mAb 证实了对IRF-3蛋白表达的沉默, α-Tubulin (11H10) Rabbit mAb作为上样量的对照。 |
