| 货号 | 13291S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM SignalSilence® IRF-7 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). |
| 存放说明 | -20C |
| 参考文献 | Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58. Au, W.C. et al. (1998) J Biol Chem 273, 29210-7. Wathelet, M.G. et al. (1998) Mol Cell 1, 507-18. Marié, I. et al. (1998) EMBO J 17, 6660-9. Sato, M. et al. (2000) Immunity 13, 539-48. Honda, K. et al. (2005) Nature 434, 772-7. Colina, R. et al. (2008) Nature 452, 323-8. Lin, R. et al. (2000) J Biol Chem 275, 34320-7. Yang, H. et al. (2003) J Biol Chem 278, 15495-504. Caillaud, A. et al. (2005) J Biol Chem 280, 17671-7. |
Western blot analysis of extracts from HT-29 cells transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® IRF-7 siRNA I #13139 (+) or SignalSilence®IRF-7 siRNA II (+). Twenty-four hours after transfection, cells were treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml, overnight; +) and analyzed by western blot using IRF-7 (D2A1J) Rabbit mAb #13014 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The IRF-7 (D2A1J) Rabbit mAb confirms silencing of IRF-7 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. |
