SignalSilence® IκBα siRNA I【科瑞奥博】

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SignalSilence® IκBα siRNA I

货号： 6327S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	6327S
描述	SignalSilence® IκBα siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit IκBα expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
反应种属	Human
应用	TFN
目标/特异性	IκBα siRNA I will inhibit human, mouse, rat and monkey IκBα expression.

性能
供应商	CST
背景	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
存放说明	-20C
参考文献	Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. Brown, K. et al. (1995) Science 267, 1485-8. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. Chen, Z.J. et al. (1996) Cell 84, 853-62. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

参考图片

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® IκBα siRNA I (+), using IκBα Antibody #9242 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The IκBα Antibody confirms silencing of IκBα expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western免疫印迹。用IκBα Antibody #9242 (上图) 或α-Tubulin (11H10) Rabbit mAb #2125 (下图) 研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或 SignalSilence® IκBα siRNA I (+) 的HeLa细胞的细胞提取液。IκBα Antibody 证实了对IκBα蛋白表达的沉默, α-Tubulin (11H10) Rabbit mAb作为上样量的对照。