| 货号 | 8650S |
| 描述 | SignalSilence® LRP6 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit LRP6 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/β-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1 (8-10). Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling (8-10). |
| 存放说明 | -20C |
| 参考文献 | Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248, 879-888. Pinson, K.I. et al. (2000) Nature 407, 535-538. Tamai, K. et al. (2000) Nature 407, 530-535. Kelly, O.G. et al. (2004) Development 131, 2803-2815. He, X. et al. (2004) Development 131, 1663-1677. Semënov, M.V. et al. (2001) Curr Biol 11, 951-61. Bafico, A. et al. (2001) Nat. Cell Biol. 3, 683-668. Tamai, K. et al. (2004) Mol. Cell 13, 149-156. Zeng, X. et al. (2005) Nature 438, 873-877. Davidson, G. et al. (2005) Nature 438, 867-872. |
Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® LRP6 siRNA I (+) or SignalSilence® LRP6 siRNA II #8623 (+), using LRP6 (C5C7) Rabbit mAb #2560 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The LRP6 (C5C7) Rabbit mAb confirms silencing of LRP6 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.使用LRP6 (C5C7) 兔单抗 #2560 (上) 或α-Tubulin (11H10) 兔单抗 #2125 (下)对 转染了100 nM SignalSilence®对照siRNA (Unconjugated) #6568 (-), SignalSilence® LRP6 siRNA I (+) 或SignalSilence® LRP6 siRNA II #8623 (+)的293细胞提取物进行western blot实验。LRP6 (C5C7)兔单抗用于确认LRP6的敲除，α-Tubulin (11H10) 兔单抗用于上样对照。 |
