| 货号 | 6426S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100nM MEK1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | Crews, C.M. et al. (1992) Science 258, 478-480. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19. Rosen, L.B. et al. (1994) Neuron 12, 1207-21. Cowley, S. et al. (1994) Cell 77, 841-52. Castro-Obregón, S. et al. (2004) J Biol Chem 279, 17543-53. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® MEK1 siRNA I or SignalSilence® MEK1 siRNA II #6530 (+), using MEK1 (61B12) Mouse mAb #2352 and α-Tubulin (11H10) Rabbit mAb #2125. The MEK1 (61B12) Mouse mAb confirms silencing of MEK1 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of MEK1 siRNA. |
