| 货号 | 6431S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100nM MEK2 siRNA 48 to 72 hours prior to cell lysis. See Protocol for transfection procedure. |
| 供应商 | CST |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
| 存放说明 | -20C |
| 参考文献 | Crews, C.M. et al. (1992) Science 258, 478-480. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19. Rosen, L.B. et al. (1994) Neuron 12, 1207-21. Cowley, S. et al. (1994) Cell 77, 841-52. Castro-Obregón, S. et al. (2004) J Biol Chem 279, 17543-53. |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. | |
将SignalSilence® Control siRNA (Fluorescein Conjugate) #6201转染进HeLa细胞，24h后进行荧光检测。结果证明有接近100%的转染效率。 |
