| 货号 | 6403S |
| 反应种属 | Mouse |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM SignalSilence® MEK2 siRNA II (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII. |
| 存放说明 | -20C |
| 参考文献 | Crews, C.M. et al. (1992) Science 258, 478-480. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-19. Rosen, L.B. et al. (1994) Neuron 12, 1207-21. Cowley, S. et al. (1994) Cell 77, 841-52. |
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® MEK2 siRNA I (Mouse Specific) #6402 (+), or SignalSilence® MEK2 siRNA II (Mouse Specific) (+) using MEK2 (13E3) Rabbit mAb #9147 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The MEK2 (13E3) Rabbit mAb confirms silencing of MEK2 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. |
