| 货号 | 6618S |
| 描述 | SignalSilence® Met siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Met expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 参考文献 | Cooper, C.S. et al. (1984) Nature 311, 29-33. Bottaro, D.P. et al. (1991) Science 251, 802-4. Bardelli, A. et al. (1997) Oncogene 15, 3103-11. Taher, T.E. et al. (2002) J Immunol 169, 3793-800. Schaeper, U. et al. (2000) J Cell Biol 149, 1419-32. Eder, J.P. et al. (2009) Clin Cancer Res 15, 2207-14. Sattler, M. and Salgia, R. (2009) Update Cancer Ther 3, 109-118. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Met siRNA I (+) or SignalSilence® Met siRNA II #6622 (+), using Met (25H2) Mouse mAb #3127 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Met (25H2) Mouse mAb confirms silencing of Met expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Met siRNA.用Met (25H2) Mouse mAb #3127(上)和抗α-Tubulin的兔源单克隆抗体 (11H10) #2125（下）对经过以下处理的Hela细胞进行免疫印迹检测：100 nM SignalSilence® Control siRNA(未结合的) #6568 (-)，SignalSilence® Met siRNA I (+)或者SignalSilence® Met siRNA II #6622 (+)转染的 HeLa细胞。Met (25H2) Mouse mAb确定了对Met表达的沉默作用，而抗α-Tubulin的兔源单克隆抗体作为对照确定上样量和Met siRNA的特异性。 |
