| 货号 | 13303S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM SignalSilence® Mitofusin-1 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow the protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | Mitofusins are mitochondrial transmembrane GTPases that function to regulate mitochondrial fusion, a process that occurs in concert with mitochondrial division and is necessary for the maintenance of structural and genetic mitochondrial integrity (1,2). Two mitofusins have been described in mammals, mitofusin-1 and -2, which share 60% amino acid identity and appear to function coordinately to regulate mitochondrial fusion (3). Mitochondrial fusion is widely recognized as important for normal cell growth and development (4), and may have evolved as a mechanism to offset the deleterious effects of mtDNA mutations (3). Null mutations in either mitofusin are embryonic lethal in mice, whereas conditional knockout studies have shown that combined deletion of mitofusin-1 and mitofusin-2 in skeletal muscle results in severe mitochondrial dysfunction (3). |
| 存放说明 | -20C |
| 参考文献 | Zhang, Y. and Chan, D.C. (2007) FEBS Lett 581, 2168-73. Chan, D.C. (2006) Annu Rev Cell Dev Biol 22, 79-99. Chen, H. et al. (2010) Cell 141, 280-9. Bereiter-Hahn, J. and Vöth, M. (1994) Microsc Res Tech 27, 198-219. |
Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (unconjugated) #6568 (-), SignalSilence® Mitofusin-1 siRNA I #13274 (+) or SignalSilence® Mitofusin-1 siRNA II (+), using Mitofusin-1 Antibody #13196 (upper) or β-Actin (D8A6) Rabbit mAb #8457 (lower). The Mitofusin-1 Antibody confirms silencing of mitofusin-1 expression, while the β-Actin (D8A6) Rabbit mAb is used as a loading control. 使用Mitofusin-1 Antibody #13196 (上) 或 β-Actin (D8A6) Rabbit mAb #8457 (下)对转染了SignalSilence® Control siRNA (unconjugated) #6568 (-), SignalSilence® Mitofusin-1 siRNA I #13274 (+) 或SignalSilence®Mitofusin-1 siRNA II (+)的HeLa细胞提取物进行western blot分析。Mitofusin-1 Antibody用于确认mitofusin-1的敲除，β-Actin (D8A6) Rabbit mAb兔单抗用作上样对照。 |
