| 货号 | 6499S |
| 描述 | SignalSilence® Moesin siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit moesin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). |
| 存放说明 | -20C |
| 参考文献 | Tsukita, S. and Yonemura, S. (1999) J Biol Chem 274, 34507-10. Mangeat, P. et al. (1999) Trends Cell Biol 9, 187-92. Matsui, T. et al. (1998) J Cell Biol 140, 647-57. Gautreau, A. et al. (2000) J Cell Biol 150, 193-203. Tran Quang, C. et al. (2000) EMBO J 19, 4565-76. Gautreau, A. et al. (1999) Proc Natl Acad Sci U S A 96, 7300-5. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Moesin siRNA I (+), using Moesin (Q480) Antibody #3150 and α-Tubulin (11H10) Rabbit mAb #2125. The Moesin (Q480) Antibody confirms silencing of moesin expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. |
