| 货号 | 6284S |
| 描述 | SignalSilence® MTAP siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit MTAP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | MTAP is an enzyme that is essential for the salvage pathway for both adenine and methionine synthesis. MTAP catalyzes the cleavage of 5’-methylthioadenosine into adenine and 5-methylthio-D-ribose-1-phosphate. Adenine is then used to generate AMP whereas 5-methylthio-D-ribose-1-phosphate is converted into methionine (1,2). MTAP is expressed in all normal cells and tissues, although frequently lost in different human tumors including pancreatic adenocarcinoma, neuroendocrine tumors, non-small cell lung carcinoma and breast carcinoma. MTAP is usually codeleted with p16 (cdkN2a/ARF) (3-5). MTAP overexpression in breast cancer cells inhibits their ability to form colonies in soft agar, thereby implicating its function as a tumor suppressor (6). |
| 存放说明 | -20C |
| 参考文献 | Backlund, P.S. and Smith, R.A. (1981) J Biol Chem 256, 1533-5. Backlund, P.S. et al. (1982) J Biol Chem 257, 4196-202. Dreyling, M.H. et al. (1998) Genes Chromosomes Cancer 22, 72-8. Zhang, H. et al. (1996) Cancer Genet Cytogenet 86, 22-8. Illei, P.B. et al. (2003) Clin Cancer Res 9, 2108-13. Christopher, S.A. et al. (2002) Cancer Res 62, 6639-44. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® MTAP siRNA I (+) or SignalSilence® MTAP II #6285 (+), using MTAP Antibody #4158 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The MTAP Antibody confirms silencing of MTAP expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot检测hela细胞提取物，细胞用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® MTAP siRNA I (+)或SignalSilence® MTAP II #6285 (+)，检测抗体为MTAP Antibody #4158 (upper)或α-Tubulin (11H10) Rabbit mAb #2125 (lower)。MTAP Antibody用于验证MTAP基因的沉默效果，α-Tubulin (11H10) Rabbit mAb兔单抗是内参对照。 |
