| 货号 | 6381S |
| 描述 | SignalSilence® mTOR siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit mTOR expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® mTOR siRNA I will inhibit human, mouse and rat mTOR expression. |
| 供应商 | CST |
| 背景 | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). |
| 存放说明 | -20C |
| 参考文献 | Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22. Brown, E.J. et al. (1994) Nature 369, 756-8. Sabatini, D.M. et al. (1994) Cell 78, 35-43. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26. Dennis, P.B. et al. (2001) Science 294, 1102-5. Fang, Y. et al. (2001) Science 294, 1942-5. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7. Kim, D. H. et al. (2002) Cell 110, 163-175. |
Western blot analysis of extracts from HeLa cells, transfected with either control siRNA (-) or SignalSilence® mTOR siRNA I (+), using mTOR Antibody #2972 and eIF4B Antibody #3592. The mTOR antibody confirms silencing of mTOR expression and eIF4B antibody is used to control for loading and specificity of mTOR siRNA. Western blot 分析HeLa细胞提取物，转染采用control siRNA (-) 或 SignalSilence® mTOR siRNA I (+)，所用抗体为mTOR Antibody #2972 或 eIF4B Antibody #3592。mTOR antibody验证了mTOR表达的沉默，eIF4B antibody作为上样对照和mTOR siRNA的特异性验证。 | |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. 荧光检测转染24小时后的Hela细胞，采用SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 进行转染，显示100% 的转染效率。 |
