SignalSilence® MUC1 siRNA I【科瑞奥博】

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SignalSilence® MUC1 siRNA I

货号： 13253S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	13253S
反应种属	Human
应用	TFN
使用方法	CST recommends transfection with 100 nM SignalSilence^® MUC1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

性能
供应商	CST
背景	Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a cytoplasmic tail of 72 residues (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ and Src through phosphorylation at Ser44, Thr41 and Tyr46 of MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6), and attenuates stress-induced apoptosis through the Akt or p53 pathways (7,8).
存放说明	-20C
参考文献	Baldus, S.E. et al. (2004) Crit Rev Clin Lab Sci 41, 189-231. Schroeder, J.A. et al. (2001) J Biol Chem 276, 13057-64. Li, Y. et al. (1998) Mol Cell Biol 18, 7216-24. Li, Y. et al. (2001) J Biol Chem 276, 6061-4. Ren, J. et al. (2002) J Biol Chem 277, 17616-22. Schroeder, J.A. et al. (2004) Oncogene 23, 5739-47. Raina, D. et al. (2004) J Biol Chem 279, 20607-12. Wei, X. et al. (2005) Cancer Cell 7, 167-78.

参考图片

Western blot analysis of extracts from ZR-75-1 cells, transfected with 100 nM SignalSilence^® Control siRNA (Unconjugated) #6568 (-) or SignalSilence^® MUC1 siRNA I (+), using MUC1 (VU4H5) Mouse mAb #4538 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The MUC1 (VU4H5) Mouse mAb confirms silencing of MUC1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.使用MUC1 (VU4H5) Mouse mAb #4538（上）或SignalSilence® MUC1 siRNA I（下）对转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-),或SignalSilence® MUC1 siRNA I (+)的ZR-75-1细胞提取物进行western blot分析。MUC1 (VU4H5) Mouse mAb用于确认MUC1被敲除，β-Actin (D6A8) Rabbit mAb用于确认上样量一致。