| 货号 | 8964S |
| 描述 | SignalSilence® NDP52 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit NDP52 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | NDP52 (CALCOCO2) is ubiquitously expressed and composed of an amino-terminal SKICH domain, followed by a coiled-coil domain, and two zinc finger domains (1). It has recently been reported to act as an autophagy receptor that binds cytosolic ubiquitinated bacteria, leading to autophagy activation and pathogen clearance (1-3). NDP52 binds ubiquitin through its zinc finger domain and simultaneously binds LC3, which directs the bacteria into autophagosomes. In addition, NDP52 interacts with Nap1 and SINTBAD to recruit TBK1 to ubiquitinated bacteria (1). |
| 存放说明 | -20C |
| 参考文献 | Thurston, T.L. et al. (2009) Nat Immunol 10, 1215-21. Cemma, M. et al. (2011) Autophagy 7, 341-5. Mostowy, S. et al. (2011) J Biol Chem 286, 26987-95. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® NDP52 siRNA I (+), or SignalSilence® NDP52 siRNA II #9000 (+), using NDP52 Antibody #9036 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The NDP52 Antibody confirms silencing of NDP52 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.Western blot分析HeLa细胞的细胞提取物，转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® NDP52 siRNA I (+), or SignalSilence® NDP52 siRNA II #9000 (+)，使用抗体是NDP52 Antibody #9036 (上图)或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。NDP52 Antibody证实NDP52表达的沉默，β-Actin (D6A8) Rabbit mAb用来检测内参。 |
