SignalSilence® NF-κB p65 siRNA I【科瑞奥博】

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SignalSilence® NF-κB p65 siRNA I

货号： 6261S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	6261S
描述	SignalSilence® NF-kappaB p65 siRNA from Cell Signaling Technology allows the researcher to specifically inhibit NF-kappaB p65 expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce protein expression.
反应种属	Human
应用	TFN

性能
供应商	CST
背景	Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
存放说明	-20C
计算分子量	65
参考文献	Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. Haskill, S. et al. (1991) Cell 65, 1281-9. Thompson, J.E. et al. (1995) Cell 80, 573-82. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. Chen, Z.J. et al. (1996) Cell 84, 853-62. Senftleben, U. et al. (2001) Science 293, 1495-9. Coope, H.J. et al. (2002) EMBO J 21, 5375-85. Xiao, G. et al. (2001) Mol Cell 7, 401-9. Verma, U. N. et al. (2003) Clin. Cancer Res. 9, 1291-1300.

参考图片

Western blot analysis of extracts from HeLa cells transfected with nontargeted (-) or targeted (+) siRNA. p65 was detect using NF-kappaB p65 Antibody #3034, and p42 was detected using p42 MAPK Antibody #9108. The NF-kappaB p65 Antibody confirms silencing of p65 expression, and the p42 MAPK Antibody is used to control for loading and siRNA specificity.Western免疫印迹研究转染了nontargeted (-) 或 targeted (+) siRNA的 HeLa细胞的细胞抽提液。检测p65用NF-kappaB p65 Antibody #3034, 检测p42 用p42 MAPK Antibody #9108。NF-kappaB p65 Antibody 证实了p65 蛋白的表达,p42 MAPK Antibody用作siRNA特异性的对照。

Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.荧光检测转染了SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 24小时后的活的HeLa，证明了转染的效率接近100%。