| 货号 | 6337S |
| 描述 | SignalSilence® NF-κB p65 siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit NF-κB p65 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® NF-κB p65 siRNA I (Mouse Specific) inhibits mouse and rat NF-κB p65 expression. |
| 供应商 | CST |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | -20C |
| 参考文献 | Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. Haskill, S. et al. (1991) Cell 65, 1281-9. Thompson, J.E. et al. (1995) Cell 80, 573-82. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. Chen, Z.J. et al. (1996) Cell 84, 853-62. Senftleben, U. et al. (2001) Science 293, 1495-9. Coope, H.J. et al. (2002) EMBO J 21, 5375-85. Xiao, G. et al. (2001) Mol Cell 7, 401-9. |
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® NF-κB p65 siRNA I (Mouse Specific) (+), or SignalSilence® NF-κB p65 siRNA II (Mouse Specific) #6339 (+), using NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The NF-κB p65 (D14E12) XP® Rabbit mAb confirms silencing of NF-κB p65 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.Western免疫印迹。用NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (上图) 或α-Tubulin (11H10) Rabbit mAb #2125 (下图)研究转染了100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® NF-κB p65 siRNA I (Mouse Specific) (+), 或SignalSilence® NF-κB p65 siRNA II (Mouse Specific) #6339 (+)的NIH/3T3细胞的细胞抽提液。The NF-κB p65 (D14E12) XP® Rabbit mAb 证实了对NF-κB p65 蛋白表达的沉默，以α-Tubulin (11H10) Rabbit mAb 作为对照。 |
