| 货号 | 6558S |
| 描述 | SignalSilence® p21 Waf1/Cip1 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit p21 Waf1/Cip1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.CST的SignalSilence® p21 Waf1/Cip1 siRNA II使得研究人员可以使用RNA干扰的方法特异性抑制p21 Waf1/Cip1的表达，RNA干扰是一种通过将双链RNA分子转入细胞从而选择性沉默基因表达的方法。CST的所有SignalSilence® siRNA产品都通过了严格的内部测试，并使用western blot方法检测其减少目标蛋白表达的效用。 |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A is active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).肿瘤抑制蛋白p21 Waf1/Cip1能够充当细胞周期进程抑制剂。其功能与周期蛋白和周期蛋白依赖性激酶形成的异源三聚体存在化学计量关系。通过与CDK2复合体联合，它可以抑制激酶的活性和阻断G1/S进程(1)。然而，p21也可以增强CDK4或者CDEK6与周期蛋白-D形成的复合体的组装和活性(2)。p21的羧基末端区域能够充分结合并抑制PCNA，DNA聚合酶的一个亚基，而且可以协调细胞周期进程中DNA的复制(3)。基于紫外线损伤或者是在细胞周期中cdc2/cyclin B或CDK2/cyclin A活跃阶段，p53被磷酸化且通过p53-响应元件上调p21的表达(4)。泛素化和蛋白酶体降解能够下调p21的蛋白水平(5)。 |
| 存放说明 | -20C |
| 参考文献 | Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-534. Cheng, J. et al. (1999) EMBO J. 18, 1571-1583. Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659. Wang, Y. and Prives, C. (1995) Nature 376, 88-91. Sheaff, R.J. et al. (2000) Cell 5, 403-410. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® p21 Waf1/Cip1 siRNA II (+), using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 and α-Tubulin (11H10) Rabbit mAb #2125. The p21 Waf1/Cip1 (12D1) Rabbit mAb confirms silencing of p21 Waf1/Cip1 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p21 Waf1/Cip1 siRNA.Western blot方法分析Hela细胞提取物。分别用100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)或 SignalSilence® p21 Waf1/Cip1 siRNA II (+)转染HeLa细胞。使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb #2947和α-Tubulin (11H10) Rabbit mAb #2125。和对照抗体α-Tubulin (11H10) Rabbit mAb #2125相比，p21 Waf1/Cip1 (12D1) Rabbit mAb的检测结果证实p21 Waf1/Cip1的表达被p21 Waf1/Cip1 siRNA.特异性沉默。 | |
Immunohistochemical analysis of paraffin-embedded HeLa cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (upper) or SignalSilence® p21 Waf1/Cip1 siRNA II #6558 (lower) using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947.免疫组织化学方法检测石蜡包埋HeLa细胞，分别转染 SignalSilence® Control siRNA (Unconjugated) #6568 （左图）和SignalSilence® p21 Waf1/Cip1 siRNA II #6558 (右图)，使用的抗体为p21 Waf1/Cip1 (12D1) Rabbit mAb #2947。 |
