| 货号 | 12324S |
| 描述 | SignalSilence® p27 Kip1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit p27 Kip1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.Cell Signaling Technology (CST) 公司的SignalSilence® p27 Kip1 siRNA I 帮助研究人员使用RNA干扰技术特异性抑制p27 Kip1表达，该技术通过向细胞内导入双链RNA分子以选择性的抑制某些基因的表达。所有CST公司的SignalSilence® siRNA都经过了严格的内部测试，western分析显示能够能够降低目标蛋白的表达。Quality Control |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).p27 Kip1是细胞周期蛋白激酶抑制因子Cip/Kip家族的一员。与它的同类蛋白类似，p57 Kip2 和p21 Waf1/Cip1，它实施G1限制点的能力来自于阻止和CDK2/cyclin E和其他CDK/cyclin复合物结合。p27的表达水平在休眠细胞和被cAMP或其他细胞周期负调因子处理过的细胞中表达上调。白介素2或其他有丝分裂原处理后能够下调p27；这一机制设计了p27的磷酸化，p27的降解是通过泛素-蛋白酶体通路进行的（1-4）。 |
| 存放说明 | -20C |
| 参考文献 | Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23. Polyak, K. et al. (1994) Genes Dev 8, 9-22. Kato, J.Y. et al. (1994) Cell 79, 487-96. Vlach, J. et al. (1997) EMBO J 16, 5334-44. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® p27 Kip1 siRNA I (+), or SignalSilence® p27 Kip1 siRNA II #12410 (+), using p27 Kip1 (D69C12) XP® Rabbit mAb #3686 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The p27 Kip1 (D69C12) XP® Rabbit mAb confirms silencing of p27 Kip1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.转染100 nM SignalSilence®对照siRNA(未标记) #6568 (-),SignalSilence® p27 Kip1 siRNA I (+),或SignalSilence® p27 Kip1 siRNA II #12410 (+)的HeLa细胞提取物，使用p27 Kip1 (D69C12) XP® Rabbit mAb #3686 (上)或β-Actin (D6A8) Rabbit mAb #8457 (下)进行western blot分析。p27 Kip1 (D69C12) XP® Rabbit mAb可以确认p27 Kip1表达受抑制，β-Actin (D6A8) Rabbit mAb用于确认上样量一致。 |
