| 货号 | 6279S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM p38β MAPK siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 参考文献 | Rouse, J. et al. (1994) Cell 78, 1027-37. Han, J. et al. (1994) Science 265, 808-11. Lee, J.C. et al. (1994) Nature 372, 739-46. Freshney, N.W. et al. (1994) Cell 78, 1039-49. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6. Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4. Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30. Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31. |
Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® p38β MAPK siRNA I #6278 (+) or SignalSilence® p38β MAPK siRNA II (+), using p38β MAPK (C28C2) Rabbit mAb #2339 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The p38β MAPK (C28C2) Rabbit mAb confirms silencing of p38β MAPK expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.western blot方法检测细胞提取物：转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)和SignalSilence®p38β MAPK siRNA I #6278 (+)、 SignalSilence®p38β MAPK siRNA II (+)的293 细胞，使用的抗体是p38β MAPK (C28C2) Rabbit mAb #2339（上图） 和α-Tubulin (11H10) Rabbit mAb #2125（下图）。用 p38β MAPK (C28C2) Rabbit mAb证实了p38β MAPK的沉默表达，而α-Tubulin (11H10) Rabbit mAb被用于负载对照。 |
