SignalSilence® PAK1 siRNA I (Mouse Specific)【科瑞奥博】

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SignalSilence® PAK1 siRNA I (Mouse Specific)

货号： 8093S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	8093S
描述	SignalSilence® PAK1 siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PAK1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
反应种属	Mouse
应用	TFN

性能
供应商	CST
背景	The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1 and Ser192 and Ser197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). PAK4, PAK5, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10).
存放说明	-20C
参考文献	Knaus, U.G. and Bokoch, G.M. (1998) Int. J. Biochem. Cell Biol. 30, 857-862. Daniels, R.H. et al. (1998) EMBO J. 17, 754-764. King, C.C. et al. (2000) J. Biol. Chem. 275, 41201-41209. Manser, E. et al. (1997) Mol. Cell. Biol. 17, 1129-1143. Gatti, A. et al. (1999) J. Biol. Chem. 274, 8022-8028. Lei, M. et al. (2000) Cell 102, 387-397. Chong, C. et al. (2001) J. Biol. Chem. 276, 17347-17353. Zhao, Z. et al. (2000) Mol. Cell. Biol. 20, 3906-3917. Abo, A. et al. (1998) EMBO J. 17, 6527-6540. Qu, J. et al. (2001) Mol. Cell. Biol. 21, 3523-3533.

参考图片

Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® PAK1 siRNA I (Mouse Specific) (+), using PAK1 Antibody #2602 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The PAK1 Antibody confirms silencing of PAK1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

使用PAK1 Antibody #2602 (上图)和α-Tubulin (11H10) Rabbit mAb #2125 (下图)，免疫印迹（Western blot）分析HeLa细胞中PAK1蛋白水平，细胞分别转染100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® PAK1 siRNA I (Mouse Specific) (+)。PAK1 antibody确定PAK1基因沉默，然而α-Tubulin (11H10) Rabbit mAb通常用于内参照。