| 货号 | 6304S |
| 描述 | SignalSilence® PARP siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PARP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. CST公司的 SignalSilence ® PARP siRNA I产品能够满足研究者通过RNA干扰方式特异性抑制PARP基因表达的需求，这种方法通过将双链RNA分子释放到细胞内来实现基因表达的选择性沉默。所有CST的SignalSilence ® siRNA产品都是经过严格的内部测试，并已通过western方法证明了目标蛋白表达量的降低。Quality Control |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | PARP siRNA I will inhibit human, mouse and rat PARP expression. |
| 供应商 | CST |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). PARP，分子量116kDa，是核poly (ADP-ribose) polymerase，参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3)，在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解，裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力；PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6）。 |
| 存放说明 | -20C |
| 参考文献 | Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. Cohen, G.M. (1997) Biochem. J. 326, 1-16. Nicholson, D. W. et al. (1995) Nature 376, 37-43. Tewari, M. et al. (1995) Cell 81, 801-809. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® PARP siRNA I (+) or SignalSilence® PARP siRNA II #6305 (+), using PARP (46D11) Rabbit mAb #9532 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The PARP (46D11) Rabbit mAb confirms silencing of PARP expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot方法检测HeLa细胞提取物：用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® PARP siRNA I (+)或SignalSilence® PARP siRNA II #6305 (+)转染；使用PARP (46D11) Rabbit mAb #9532 (上图) 或α-Tubulin (11H10) Rabbit mAb #2125 (下图)检测。PARP (46D11) Rabbit mAb确定PARP表达的沉默，α-Tubulin (11H10) Rabbit mAb作为内参对照使用。 |
