SignalSilence® PARP siRNA I (Mouse Specific)【科瑞奥博】

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SignalSilence® PARP siRNA I (Mouse Specific)

货号： 6409S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	6409S
描述	SignalSilence® PARP siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PARP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
反应种属	Mouse
应用	TFN

性能
供应商	CST
存放说明	-20C
参考文献	Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. Cohen, G.M. (1997) Biochem. J. 326, 1-16. Nicholson, D. W. et al. (1995) Nature 376, 37-43. Tewari, M. et al. (1995) Cell 81, 801-809. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

参考图片

Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence^® Control siRNA (Unconjugated) #6568 (-), SignalSilence^® PARP siRNA I (Mouse Specific) (+), or SignalSilence^® PARP siRNA II (Mouse Specific) #6412 (+) using PARP (46D11) Rabbit mAb #9532 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The PARP (46D11) Rabbit mAb confirms silencing of PARP expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Western blot方法检测NIH/3T3细胞提取物, 用100 nM SignalSilence^® Control siRNA (Unconjugated) #6568 (-)，SignalSilence^® PARP siRNA I (Mouse Specific) (+)或SignalSilence^® PARP siRNA II(Mouse Specific) #6412 (+)转染；使用PARP (46D11) Rabbit mAb #9532 (上图) 或α-Tubulin (11H10) Rabbit mAb #2125 (下图)检测。PARP (46D11) Rabbit mAb确定PARP表达的沉默，α-Tubulin (11H10) Rabbit mAb作为内参对照使用。