| 货号 | 6412S |
| 描述 | SignalSilence® PARP siRNA II (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PARP expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Mouse |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | -20C |
| 参考文献 | Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. Cohen, G.M. (1997) Biochem. J. 326, 1-16. Nicholson, D. W. et al. (1995) Nature 376, 37-43. Tewari, M. et al. (1995) Cell 81, 801-809. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539. |
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® PARP siRNA I #6409 (Mouse Specific) (+), or SignalSilence® PARP siRNA II (Mouse Specific) (+) using PARP (46D11) Rabbit mAb #9532 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The PARP (46D11) Rabbit mAb confirms silencing of PARP expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot方法检测NIH/3T3细胞提取物，用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)，SignalSilence® PARP siRNA I (Mouse Specific) #6409 (+)或SignalSilence® PARP siRNA II(Mouse Specific)(+)转染细胞；使用PARP (46D11) Rabbit mAb #9532 (上图) 或α-Tubulin (11H10) Rabbit mAb兔单抗#2125 (下图)检测。PARP (46D11) Rabbit mAb兔单抗确定PARP表达的沉默，α-Tubulin (11H10) Rabbit mAb作为内参对照使用。 |
