| 货号 | 6314S |
| 描述 | SignalSilence® PDK1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PDK1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis. |
| 存放说明 | -20C |
| 参考文献 | Belham, C. et al. (1999) Curr. Biol. 9, R93-R96. Toker, A. and Newton, A.C. (2000) Cell 103, 185-188. Williams, M.R. et al. (2000) Curr. Biol. 10, 439-448. |
Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® PDK1 siRNA I (+), using PDK1 (D37A7) Rabbit mAb #5662 and α-Tubulin (11H10) Rabbit mAb #2125. The PDK1 (D37A7) Rabbit mAb confirms silencing of PDK1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot 分析K-562细胞提取物，转染采用100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) 或 SignalSilence ® PDK1 siRNA I (+) ，所用抗体为PDK1 (D37A7) Rabbit mAb 兔单抗#5662 和 α-Tubulin (11H10) Rabbit mAb兔单抗 #2125 。 PDK1 (D37A7) Rabbit mAb兔单抗验证了 GSK-3β表达的沉默α-Tubulin (11H10) Rabbit mAb兔单抗作为上样对照。 |
