| 货号 | 9026S |
| 描述 | SignalSilence® PERK siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PERK expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status. |
| 存放说明 | -20C |
| 参考文献 | Harding, H. et al. (1999) Nature 397, 271-274. Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509. Harding, H. et al. (2001) Mol. Cell 7, 1153-1163. |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® PERK siRNA I #9024 (+), or SignalSilence® PERK siRNA II (+), using PERK (C33E10) Rabbit mAb #3192 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The PERK (C33E10) Rabbit mAb confirms silencing of PERK expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. Western blot 方法检测293T细胞提取物。用100 nM SignalSilence® Control siRNA (未接合的) #6568 (-)，SignalSilence® PERK siRNA I #9024 (+)或SignalSilence® PERK siRNA II (+)转染细胞。所用抗体为 PERK (C33E10) Rabbit mAb兔单抗#3192（上图）或β-Actin (D6A8) Rabbit mAb #8457（下图）。PERK (C33E10) Rabbit mAb兔单抗确定PERK 表达的沉默效率， β-Actin (D6A8) Rabbit mAb兔单抗作为上样量对照。 |
