SignalSilence® PI3 Kinase p110β siRNA I【科瑞奥博】

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SignalSilence® PI3 Kinase p110β siRNA I

货号： 13364S 基本售价： 3318.0 元规格： 300ul

产品信息

概述
货号	13364S
反应种属	Human
应用	TFN
使用方法	CST recommends transfecting with 100 nM PI3 Kinase p110β siRNA I 48 to 72 hours before lysing cells. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well. CST建议转染100 nM SignalSilence® PI3 Kinase p110β siRNA I，48-72小时后裂解细胞。转染的步骤请参阅转染试剂的说明书。关于使用问题，可以随时咨询CST。每管试剂能进行100次转染，该转染次数以总体积为300μl 的1个24孔板孔中siRNA终浓度100 nM来进行计算。

性能
供应商	CST
存放说明	-20C
参考文献	Cantley, L.C. (2002) Science 296, 1655-7. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80. Stoyanov, B. et al. (1995) Science 269, 690-3. Okkenhaug, K. and Vanhaesebroeck, B. (2003) Nat Rev Immunol 3, 317-30. Czauderna, F. et al. (2003) Nucleic Acids Res 31, 670-82. Bénistant, C. et al. (2000) Oncogene 19, 5083-90. Knobbe, C.B. and Reifenberger, G. (2003) Brain Pathol 13, 507-18.

参考图片

Western blot analysis of extracts from MCF7 cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® PI3 Kinase p110β siRNA I (+), using PI3 Kinase p110β (C73F8) Rabbit mAb #3011 and β-Actin (D6A8) Rabbit mAb #8457. The PI3 Kinase p110β (C73F8) Rabbit mAb antibody confirms silencing of PI3 Kinase p110β expression while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

转染SignalSilence® Control siRNA (Unconjugated) #6568 (-),或SignalSilence® PI3 Kinase p110β siRNA I (+)的MCF7细胞提取物，使用PI3 Kinase p110β (C73F8) Rabbit mAb #3011和β-Actin (D6A8) Rabbit mAb #8457进行western blot分析。PI3 Kinase p110β (C73F8) Rabbit mAb可以确认PI3 Kinase p110β被敲除，β-Actin (D6A8) Rabbit mAb兔单抗用于确认上样量一致。