| 货号 | 6912S |
| 描述 | SignalSilence® PI3 Kinase p85α siRNA I (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PI3 Kinase p85α expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Mouse |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). |
| 存放说明 | -20C |
| 参考文献 | Cantley, L.C. (2002) Science 296, 1655-7. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80. Stoyanov, B. et al. (1995) Science 269, 690-3. |
Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® PI3 Kinase p85α siRNA I (Mouse Specific) (+), using PI3 Kinase p85 (19H8) Rabbit mAb #4257 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The PI3 Kinase p85 (19H8) Rabbit mAb confirms silencing of PI3 Kinase p85α expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot 分析NIH/3T3细胞提取物，转染采用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或 SignalSilence® PI3 Kinase p85α siRNA I (Mouse Specific) (+)，所用抗体为 PI3 Kinase p85 (19H8) Rabbit mAb 兔单抗#4257 (上) 或 α-Tubulin (11H10) Rabbit mAb 兔单抗#2125 (下)。PI3 Kinase p85 (19H8) Rabbit mAb 兔单抗验证了PI3 Kinase p85α表达的沉默，α-Tubulin (11H10) Rabbit mAb兔单抗作为上样对照。 |
