SignalSilence® PKCα siRNA I
货号：
13292S 基本售价：
3318.0 元 规格：
300ul
产品信息
概述| 货号 | 13292S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM SignalSilence® PKCα siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
性能| 供应商 | CST |
| 背景 | |
| 存放说明 | -20C |
| 参考文献 | Nishizuka, Y. (1984) Nature 308, 693-8.
Keranen, L.M. et al. (1995) Curr Biol 5, 1394-403.
Mellor, H. and Parker, P.J. (1998) Biochem J 332 ( Pt 2), 281-92.
Ron, D. and Kazanietz, M.G. (1999) FASEB J 13, 1658-76.
Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Rep 1, 399-403.
Baron, C.L. and Malhotra, V. (2002) Science 295, 325-8.
Flynn, P. et al. (2000) J Biol Chem 275, 11064-70.
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参考图片Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® PKCα siRNA I (+), using PKCα Antibody #2056 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The PKCα Antibody confirms silencing of PKCα expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. |
