| 货号 | 6251S |
| 描述 | SignalSilence® PTEN siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PTEN expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® PTEN siRNA I will inhibit human, mouse, rat and monkey PTEN expression. |
| 供应商 | CST |
| 背景 | PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
| 参考文献 | Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30. Funamoto, S. et al. (2002) Cell 109, 611-23. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610. |
Western blot analysis of extracts from HeLa cells, transfected with non-targeted siRNA (-) or SignalSilence® PTEN siRNA I (+) using PTEN Antibody #9552 and p42 MAP Kinase (Erk2) Antibody #9108. The PTEN antibody confirms silencing of PTEN expression, while the p42 MAPK antibody is used to control for loading and specificity of PTEN siRNA.Western blot 分析采用non-targeted siRNA (-) or SignalSilence ® PTEN siRNA I (+)转染后的Hela细胞提取物，所用抗体为PTEN Antibody #9552 and p42 MAP Kinase (Erk2) Antibody #9108. 。 PTEN抗体确定了PTEN表达的沉默， p42 MAPK antibody作为PTEN siRNA特异性的对照。 | |
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. 在转染24小时的活的Hela细胞中，采用荧光检测SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 ，显示近100%的转染效率。 |
