| 货号 | 13348S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM SignalSilence® PTP1B siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow the protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | Protein-tyrosine phosphatase 1B (PTP1B) is the original member of the protein tyrosine phosphatase (PTP) family of proteins (1). PTP1B is highly expressed in insulin-responsive tissues and negatively regulates insulin, as well as integrin, through dephosphorylation of phosphotyrosine residues (2-4). PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes (5). |
| 存放说明 | -20C |
| 参考文献 | Liang, F. et al. (2005) J Biol Chem 280, 24857-63. Kenner, K.A. et al. (1996) J Biol Chem 271, 19810-6. Klaman, L.D. et al. (2000) Mol Cell Biol 20, 5479-89. Liu, F. et al. (1998) Curr Biol 8, 173-6. Elchebly, M. et al. (1999) Science 283, 1544-8. |
Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (unconjugated) #6568 (-), SignalSilence® PTP1B siRNA I (+) or SignalSilence® PTP1B siRNA II #13380 (+), using PTP1B Antibody #5311 (upper) or β-Actin (D8A6) Rabbit mAb #8457 (lower). The PTP1B Antibody confirms silencing of PTP1B expression, while the β-Actin (D8A6) Rabbit mAb is used as a loading control. 使用PTP1B Antibody #5311 (上) 或β-Actin (D8A6) Rabbit mAb兔单抗 #8457 (下)对转染了SignalSilence® Control siRNA (unconjugated) #6568 (-), SignalSilence® PTP1B siRNA I (+) 或SignalSilence® PTP1B siRNA II#13380 (+)的HeLa细胞提取物进行western blot分析。PTP1B抗体用于确认PTP1B的表达已经被抑制，β-Actin (D8A6) Rabbit mAb兔单抗用作上样对照。 |
