| 货号 | 13380S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM SignalSilence® PTP1B siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow the protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | Protein-tyrosine phosphatase 1B (PTP1B) is the original member of the protein tyrosine phosphatase (PTP) family of proteins (1). PTP1B is highly expressed in insulin-responsive tissues and negatively regulates insulin, as well as integrin, through dephosphorylation of phosphotyrosine residues (2-4). PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes (5). |
| 存放说明 | -20C |
| 参考文献 | Liang, F. et al. (2005) J Biol Chem 280, 24857-63. Kenner, K.A. et al. (1996) J Biol Chem 271, 19810-6. Klaman, L.D. et al. (2000) Mol Cell Biol 20, 5479-89. Liu, F. et al. (1998) Curr Biol 8, 173-6. Elchebly, M. et al. (1999) Science 283, 1544-8. |
Western blot analysis of extracts from HeLa cells, transfected with SignalSilence® Control siRNA (unconjugated) #6568 (-), SignalSilence® PTP1B siRNA I #13348 (+) or SignalSilence® PTP1B siRNA II (+), using PTP1B Antibody #5311 (upper) or β-Actin (D8A6) Rabbit mAb #8457 (lower). The PTP1B Antibody confirms silencing of PTP1B expression, while the β-Actin (D8A6) Rabbit mAb is used as a loading control. 使用PTP1B Antibody #5311 (上) 或β-Actin (D8A6) Rabbit mAb兔单抗 #8457 (下)对转染了SignalSilence® Control siRNA (unconjugated) #6568 (-), SignalSilence® PTP1B siRNA I #13348 (+) 或SignalSilence® PTP1B siRNA II (+)的HeLa细胞提取物进行western blot分析。PTP1B抗体用于确认PTP1B的表达已经被抑制，β-Actin (D8A6) Rabbit mAb兔单抗用作上样对照。 |
