| 货号 | 12845S |
| 描述 | SignalSilence® Puma siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Puma expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 目标/特异性 | SignalSilence® Puma siRNA I inhibits human and monkey Puma expression. |
| 供应商 | CST |
| 背景 | Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cyctochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suprression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4). |
| 存放说明 | -20C |
| 参考文献 | Yu, J. et al. (2001) Mol Cell 7, 673-82. Nakano, K. and Vousden, K.H. (2001) Mol Cell 7, 683-94. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74. Jeffers, J.R. et al. (2003) Cancer Cell 4, 321-8. Hemann, M.T. et al. (2004) Proc Natl Acad Sci U S A 101, 9333-8. Yu, J. et al. (2003) Proc Natl Acad Sci U S A 100, 1931-6. Villunger, A. et al. (2003) Science 302, 1036-8. |
Western blot analysis of extracts from A549 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Puma siRNA I (+), or SignalSilence® Puma siRNA II #12890 (+), using Puma (D30C10) Rabbit mAb #12450 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The Puma (D30C10) Rabbit mAb confirms silencing of Puma expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control. Western blot方法检测A549细胞提取物，100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-)、SignalSilence® Puma siRNA I (+)或SignalSilence® Puma siRNA II #12890 (+)转染；使用Puma (D30C10) Rabbit mAb 兔单抗#12450（上图）或β-Actin (D6A8) Rabbit mAb 兔单抗#8457（下图）检测。Puma (D30C10) Rabbit mAb 兔单抗确定Puma表达的沉默，β-Actin (D6A8) Rabbit mAb 兔单抗作为内参对照。 |
