| 货号 | 6542S |
| 描述 | SignalSilence® Rb siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Rb expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.CST的SignalSilence® Rb siRNA II使得研究人员可以使用RNA干扰的方法特异性抑制Rb蛋白的表达，RNA干扰是一种通过将双链RNA分子转入细胞从而选择性沉默基因表达的方法。CST的所有SignalSilence® siRNA产品都通过了严格的内部测试，并使用western blot方法检测其减少目标蛋白表达的效用。 |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).视网膜母细胞瘤肿瘤抑制蛋白Rb通过控制细胞周期的G1期限制点的进程来调控细胞增殖(1)。Rb具有3个功能特异的结合结构域并能够与转录因子家族的关键调节蛋白E2F、c-Abl酪氨酸激酶和具有保守的LXCXE模体蛋白相互作用(2-4)。CDK介导的细胞周期依赖性磷酸化能够抑制Rb靶向结合并允许细胞周期进程(5)。Rb的失活和随后的细胞周期进程似乎需要伴随周期蛋白E-CDK2磷酸化的cyclin D-CDK4/6初始磷酸化(6)。离体已经观察到不同CDK/cyclin复合物的特异性(6-8)，而且周期蛋白D1为第780位丝氨酸在体磷酸化所必需(9)。 |
| 存放说明 | -20C |
| 参考文献 | Sherr, C.J. (1996) Science 274, 1672-7. Nevins, J.R. (1992) Science 258, 424-9. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Rb siRNA I #6451 (+) or SignalSilence® Rb siRNA II (+), using Rb (4H1) Mouse mAb #9309 and α-Tubulin (11H10) Rabbit mAb #2125. The Rb (4H1) Mouse mAb confirms silencing of Rb expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Rb siRNA.Western blot方法分析Hela细胞提取物。分别用100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Rb siRNA I #6451 (+)或SignalSilence® Rb siRNA II (+)转染HeLa细胞。使用的抗体为Rb (4H1) Mouse mAb #9309和α-Tubulin (11H10) Rabbit mAb #2125。和对照抗体α-Tubulin (11H10) Rabbit mAb #2125相比，Rb (4H1) Mouse mAb的检测结果证实Rb的表达被Rb siRNA有效沉默。 |
