| 货号 | 8649S |
| 描述 | SignalSilence® Rictor siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit rictor expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis. |
| 反应种属 | Human |
| 应用 | TFN |
| 供应商 | CST |
| 背景 | Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt/PKB in vitro (2). This phosphorylation is essential for full Akt/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4). |
| 存放说明 | -20C |
| 参考文献 | Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302. Sarbassov, D.D. et al. (2005) Science 307, 1098-1101. Hresko, R.C. and Mueckler, M. (2005) J. Biol. Chem. 280, 40406-40416. Ali, S.M. and Sabatini, D.M. (2005) J. Biol. Chem. 280, 19445-19448. |
Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Rictor siRNA I (+), or SignalSilence® Rictor siRNA II #8622 (+), using Rictor (53A2) Rabbit mAb #2114 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Rictor (53A2) Rabbit mAb confirms silencing of rictor expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. Western blot 分析293细胞提取物，采用100 nM SignalSilence ® Control siRNA (Unconjugated) #6568， SignalSilence ® Rictor siRNA I (+), 或SignalSilence ® Rictor siRNA II #8622 (+) ，所用抗体为Rictor (53A2) Rabbit mAb 兔单抗#2114 (上) 或 α-Tubulin (11H10) Rabbit mAb兔单抗 #2125 (下) 。 Rictor (53A2) Rabbit mAb兔单抗验证了rictor表达的沉默， α-Tubulin (11H10) Rabbit mAb兔单抗作为上样的对照。 |
