| 货号 | 14661S |
| 反应种属 | Mouse |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM RIP3 siRNA II (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | The receptor-interacting protein (RIP) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that trigger pro-survival and inflammatory responses through the activation of NF-κB, as well as pro-apoptotic pathways (1). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and recruitment to TNF-R1 through interaction with TRADD (2,3). RIP-deficient cells show a failure in TNF-mediated NF-κB activation, making the cells more sensitive to apoptosis (4,5). RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNF-R1 signaling complex via interaction with NEMO, leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8-dependent cleavage of the RIP death domain can trigger the apoptotic activity of RIP (8). |
| 存放说明 | -20C |
| 参考文献 | Meylan, E. and Tschopp, J. (2005) Trends Biochem Sci 30, 151-9. Hsu, H. et al. (1996) Immunity 4, 387-96. Stanger, B.Z. et al. (1995) Cell 81, 513-23. Ting, A.T. et al. (1996) EMBO J 15, 6189-96. Kelliher, M.A. et al. (1998) Immunity 8, 297-303. Devin, A. et al. (2000) Immunity 12, 419-29. Zhang, S.Q. et al. (2000) Immunity 12, 301-11. Lin, Y. et al. (1999) Genes Dev 13, 2514-26. Yu, P.W. et al. (1999) Curr Biol 9, 539-42. Sun, X. et al. (1999) J Biol Chem 274, 16871-5. Zhang, D.W. et al. (2009) Science 325, 332-6. He, S. et al. (2009) Cell 137, 1100-11. Cho, Y.S. et al. (2009) Cell 137, 1112-23. Sun, L. et al. (2012) Cell 148, 213-27. |
Western blot analysis of extracts from L-929 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® RIP3 siRNA I (Mouse Specific) #14645 (+), or SignalSilence® RIP3 siRNA II (Mouse Specific) (+), using RIP3 Antibody (Rodent Specific) #14401 (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The RIP3 Antibody (Rodent Specific) confirms silencing of RIP3 expression, while the α-Actinin (D6F6) XP® Rabbit mAb is used as a loading control. |
