| 货号 | 6297S |
| 反应种属 | Human |
| 应用 | TFN |
| 使用方法 | CST recommends transfection with 100 nM RKIP siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. |
| 供应商 | CST |
| 背景 | Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-κB pathway, including NF-κB-inducing kinase (NIK), TAK1, IKKα and IKKβ (5). These interactions antagonize cytokine-induced activation of the NF-κB pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6). |
| 存放说明 | -20C |
| 参考文献 | Yeung, K. et al. (1999) Nature 401, 173-7. Yeung, K. et al. (2000) Mol Cell Biol 20, 3079-85. Corbit, K.C. et al. (2003) J Biol Chem 278, 13061-8. Lorenz, K. et al. (2003) Nature 426, 574-9. Yeung, K.C. et al. (2001) Mol Cell Biol 21, 7207-17. Fu, Z. et al. (2003) J Natl Cancer Inst 95, 878-89. |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® RKIP siRNA I #6296 (+) or SignalSilence® RKIP siRNA II (+), using RKIP (V177) Antibody #5291 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The RKIP (V177) Antibody confirms silencing of RKIP expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control. |
